Metabolic activity of Sphagnum recurvum under different environmental conditions

Marschall, Marianna and Laufer, Zsanett: Metabolic activity of Sphagnum recurvum under different environmental conditions. In: Acta biologica Szegediensis, (46) 3-4. pp. 247-248. (2002)

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Abstract

The fate of transgene is an up-to-date question in plant genetic and breeding research. For a breeder, the ability to transfer foreign gene and its Mendelian inheritance is a very important aspect. In crossing experiments the homogenous T2 transgenic line 124 was crossed with different non-transgenic females. The isolated hybrid embryos from the crosses were tested in medium containing bialaphos. This experiment showed the dominant character of bar gene. All the hybrid embryos germinated onto 5mg/l bialaphos medium expressed resistance, while the control variety (GK Góbé) showed sensitivity to the bialaphos as expected. The fertile T1 generation expressed 3:1 segregation ratio for bar +/bar- plants. The transformed foreign gene (bar) was stable in the wheat genome after two self pollinations and PAT asasays. It is therefore, possible to transform transgene successfully via sexual way into non-transgenic genotypes. Cell level selection of bar gene was possible in anther culture. The regenerated haploids and spontaneous diploids were herbicide resistant. Using in vitro haploid technique (anther culture), it’s possible to produce homogenous bar resistant wheat lines by cell-level selection.

Item Type: Article
Heading title: Proceedings of the 7th Hungarian Congress on Plant Physiology
Journal or Publication Title: Acta biologica Szegediensis
Date: 2002
Volume: 46
Number: 3-4
ISSN: 1588-385X
Page Range: pp. 247-248
Language: angol
Event Title: Hungarian Congress on Plant Physiology, 7., 2002, Szeged
Uncontrolled Keywords: Természettudomány, Biológia
Additional Information: Bibliogr.: 248. p.
Date Deposited: 2016. Oct. 17. 09:25
Last Modified: 2018. May. 28. 11:57
URI: http://acta.bibl.u-szeged.hu/id/eprint/22576

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