Genetic transformation of barley to modify expression of a 13-lipoxygenase

Sharma, Vijendra K.: Genetic transformation of barley to modify expression of a 13-lipoxygenase. Acta biologica Szegediensis, (49) 1-2. pp. 33-34. (2005)

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Immature scutella of barley were transformed with cDNA coding for a 13-lipoxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of T0 plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA. A few transgenic plants containing higher amounts of LOX-derived products have been found. These are the candidates for further analysis concerning pathogen resistance.

Item Type: Article
Event Title: Hungarian Congress on Plant Physiology, 8., 2005, Szeged, Hungarian Conference on Photosynthesis, 6., 2005, Szeged
Journal or Publication Title: Acta biologica Szegediensis
Date: 2005
Volume: 49
Number: 1-2
Page Range: pp. 33-34
ISSN: 1588-385X
Language: angol
Heading title: Cell biology; Proceedings
Uncontrolled Keywords: Természettudomány, Biológia
Additional Information: Bibliogr.: 34. p.; Abstract
Date Deposited: 2016. Oct. 17. 09:24
Last Modified: 2018. May. 28. 10:49

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