Fluorescence induction reveals organization of antenna and reaction center in photosynthetic bacteria

Gerencsér, László and Maróti, Péter: Fluorescence induction reveals organization of antenna and reaction center in photosynthetic bacteria. Acta biologica Szegediensis, (51) 1. pp. 27-32. (2007)

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Abstract

The photochemical phase of the bacteriochlorophyll fluorescence induction generated by rectangular shape of laser diode illumination was measured in different organization levels {whole cell, chromatophore and isolated reaction center protein) of carotenoidtess mutant of purple photosynthetic bacterium Rhodobacter sphaeroides R-26.1. While the antenna containing species showed large and positive variable fluorescence (fj relative to the constant (initial) fluorescence (Fa) {FJF^ ~ 4.5 in whole ceil), the isolated RC had smaller and negative change (FJF - -0.6). The variable fluorescence of the ceils increased steadily in the function of the age of the cultivated bacterium: FJF0 - 2 for young ceils and FJF0 - 4.5 fór old ceils while the rise time of the fluorescence induction remained constant (- 2 ms). In chromatophore, 7 times higher rate was measured than in isolated reaction center under identical experimental conditions. The results obtained under different conditions are interpreted by an extended version of the Lavergne-Trissl model where the simultaneously measured fluorescence inductions from the antenna and the RC can be separately expressed.

Item Type: Article
Journal or Publication Title: Acta biologica Szegediensis
Date: 2007
Volume: 51
Number: 1
Page Range: pp. 27-32
ISSN: 1588-385X
Language: angol
Heading title: Articles
Uncontrolled Keywords: Természettudomány, Biológia
Additional Information: Bibliogr.: 32. p.; Abstract
Date Deposited: 2016. Oct. 17. 09:24
Last Modified: 2018. May. 25. 16:03
URI: http://acta.bibl.u-szeged.hu/id/eprint/22814

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