Saxena Rajshree; Singh Rajni: Characterization of a metallo-protease produced in solid state fermentation by a newly isolated Bacillus strain. In: Acta biologica Szegediensis, (55) 1. pp. 13-18. (2011)
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A metalloprotease was characterised from a newly isolated Bacillus strain. The enzyme activity was stimulated by mono- and divalent metal ions at 5 and 10 mM concentration and inhibited by EDTA, DTT and B ME (95, 90 and 80%, respectively) at 10 mM concentration. The enzyme inhibition caused by 10 mM EDTA was restored by 45-47% with 5 and 10 mM NaCl. These characteristics established the enzyme as a metalloprotease. Different agroindustrial substrates were used for economic production of the metalloprotease, among which rice bran was selected as best providing z1000 U protease /g of substrate in 72 h with 20% inoculum and 1:3 moisture content (w/v). The enzyme was thermostable (30-50oC) with maximum activity at 60°C. It also exhibited a broad pH activity range (6-9) with a maximum at pH 8.
| Mű típusa: | Cikk, tanulmány, mű |
|---|---|
| Rovatcím: | Articles |
| Befoglaló folyóirat/kiadvány címe: | Acta biologica Szegediensis |
| Dátum: | 2011 |
| Kötet: | 55 |
| Szám: | 1 |
| ISSN: | 1588-385X |
| Oldalak: | pp. 13-18 |
| Nyelv: | angol |
| Befoglaló mű URL: | http://acta.bibl.u-szeged.hu/39261/ |
| Kulcsszavak: | Természettudomány, Biológia |
| Megjegyzések: | Bibliogr.: 18. p.; Abstract |
| Feltöltés dátuma: | 2016. okt. 17. 09:24 |
| Utolsó módosítás: | 2021. ápr. 13. 08:27 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/23151 |
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