Characterization of a metallo-protease produced in solid state fermentation by a newly isolated Bacillus strain

Saxena, Rajshree and Singh, Rajni: Characterization of a metallo-protease produced in solid state fermentation by a newly isolated Bacillus strain. In: Acta biologica Szegediensis, (55) 1. pp. 13-18. (2011)

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Abstract

A metalloprotease was characterised from a newly isolated Bacillus strain. The enzyme activity was stimulated by mono- and divalent metal ions at 5 and 10 mM concentration and inhibited by EDTA, DTT and B ME (95, 90 and 80%, respectively) at 10 mM concentration. The enzyme inhibition caused by 10 mM EDTA was restored by 45-47% with 5 and 10 mM NaCl. These characteristics established the enzyme as a metalloprotease. Different agroindustrial substrates were used for economic production of the metalloprotease, among which rice bran was selected as best providing z1000 U protease /g of substrate in 72 h with 20% inoculum and 1:3 moisture content (w/v). The enzyme was thermostable (30-50oC) with maximum activity at 60°C. It also exhibited a broad pH activity range (6-9) with a maximum at pH 8.

Item Type: Article
Heading title: Articles
Journal or Publication Title: Acta biologica Szegediensis
Date: 2011
Volume: 55
Number: 1
ISSN: 1588-385X
Page Range: pp. 13-18
Language: angol
Uncontrolled Keywords: Természettudomány, Biológia
Additional Information: Bibliogr.: 18. p.; Abstract
Date Deposited: 2016. Oct. 17. 09:24
Last Modified: 2018. May. 25. 11:04
URI: http://acta.bibl.u-szeged.hu/id/eprint/23151

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