Ghosh Priyanka and Das Arpan and Gayen Soumi and Mondal Keshab Chandra and Ghosh Uma: Statistical optimalization of α-Amylase production from Penicillium notatum NCIM 923 and kinetics study of the purified enzyme. In: Acta biologica Szegediensis, (59) 2. pp. 179-188. (2015)
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Abstract
In this study, response surface methodology (RSM) was employed to optimize the production of α-amylase by Penicillium notatum NCIM 923 through solid-state fermentation. The individual and combinational effects of the factors, i.e. substrate amount, initial moisture, fermentation time, temperature and size of inoculum were found to have significant effects on α-amylase production: the optimum values of the tested variables were 5 g, 70%, 94 h, 28 °C and 20%, respectively. The predicted amylase production (2819.24 U/g) was in good agreement with the value measured under optimized surrounding (2810.33 U/g). The molecular mass of purified α-amylase was about 52 kDa. The enzyme activity exhibited its pH optimum between pH 4.6 and 6.6, and it had maximal activity at 50 °C. The apparent Km and Vmax of α-amylase for starch were 4.1 mg/ml and 247.6 μmol/min, respectively. The activation energy (Ea) for starch hydrolysis was found to be 14.133 kJ/mol. The enzyme was thermostable with half-life (t1/2) of 110 min at 80 °C and temperature coefficient (Q10) value of 1.0. Purified enzyme was activated by Ca2+ and inhibited by Hg2+ ions. EDTA also inhibited the enzyme activity, indicating that the purified enzyme is a metalloenzyme.
| Item Type: | Article |
|---|---|
| Heading title: | Articles |
| Journal or Publication Title: | Acta biologica Szegediensis |
| Date: | 2015 |
| Volume: | 59 |
| Number: | 2 |
| ISSN: | 1588-385X |
| Page Range: | pp. 179-188 |
| Language: | English |
| Related URLs: | http://acta.bibl.u-szeged.hu/39273/ |
| Uncontrolled Keywords: | Penicillium, Mikrobiológia gombák |
| Additional Information: | Bibliogr.: p. 187-188. |
| Date Deposited: | 2016. Oct. 17. 10:36 |
| Last Modified: | 2021. Apr. 12. 15:10 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/35992 |
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