Comparative characterization of some fungal laccases

Sajben-Nagy, Enikő and Manczinger, László and Škrbić, Biljana and Đurišić Mladenović, N. and Vágvölgyi, Csaba: Comparative characterization of some fungal laccases. In: Review on agriculture and rural development, (1) 1. pp. 413-417. (2012)

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Abstract

Laccase producing fungi were isolated from environmental samples. They were identified on the basis of ITS (Internal Transcribed Spacer) sequence analysis. The laccase production of the isolates were investigated and compared with those of other strains deriving from the Szeged Microbiological Collection and from mushroom producer’s spawn. The enzyme production were examined in various (e.g. basic, mineral and inducer containing) liquid media. The pH optimum determinations for laccase activities were carried out in cell free ferment broths, at pH 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7 and 8, applying 25 mM succinate buffer. The investigations were based on ABTS [2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)] substrate measurement method. The results showed that under these conditions, among the ascomycetes strains investigated the best laccase producer was a Rhizoctonia solani isolate (the SzMC 6252J strain). Among the basidiomycetes tested, the best laccase producer was a Ganoderma isolate (HM3).

Item Type: Article
Journal or Publication Title: Review on agriculture and rural development
Date: 2012
Volume: 1
Number: 1
ISSN: 2063-4803
Page Range: pp. 413-417
Uncontrolled Keywords: Remediáció, Mikrobiológia, Analitikai kémia, Enzimek - analitikai kémia
Additional Information: Bibliogr.: p. 416-417.
Date Deposited: 2018. Jun. 14. 16:51
Last Modified: 2018. Jul. 26. 09:18
URI: http://acta.bibl.u-szeged.hu/id/eprint/55219

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