Isolation of microbes for the bioaugmentation of pollutants from river water samples

Hatvani, Lóránt and Manczinger, László and Bajkán, Szilvia and Vidács, Lívia and Radulov, Isidora and Niţǎ, Lucian Dimitru and Vágvölgyi, Csaba: Isolation of microbes for the bioaugmentation of pollutants from river water samples. Review on agriculture and rural development, (2) 1. pp. 234-238. (2013)

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Abstract

A wide range of chemical pollutants occur in rivers, several of which may impair human health severely. The objectives of this study were the isolation, identification and characterization of xenobiotic-degrading microbes from ten different locations of the Romanian and Hungarian parts of River Maros in an international collaboration. High bacterial and fungal diversity was revealed by RISA (ribosomal intergenic spacer analysis) studies. Microbes were isolated from the water samples on media containing 1 mg/ml acetanilide, aniline-HCl, 2,6-dimethylaniline, 4-isopropylaniline, chlorpropham, diuron, Na-benzoate, 3,4- dihydroxybenzoate, 4-hydroxybenzoate, methylparaben, phenol, m-cresol, p-cresol, resorcinol, phenoxiacetic acid and 2,4-dichlorophenoxiacetic acid separately. The isolates were deposited in the Pollutant-Degrading Microorganism Collection (PDMC) of the University of Szeged. The degradation of acetanilide was monitored by spectrophotometry and the three best degraders were all identified as Rhodococcus erythropolis. The xenobiotic-degrading microbes isolated in this study might be used for bioaugmentation purposes.

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