Application of laccases, produced by ganoderma species, for the detoxification of some aniline and phenol derivatives

Sajben-Nagy, Enikő and Manczinger, László and Škrbić, Biljana and Živančev, Jelena and Antić, Igor and Krisch, Judit and Vágvölgyi, Csaba: Application of laccases, produced by ganoderma species, for the detoxification of some aniline and phenol derivatives. Review on agriculture and rural development, (2) 1. pp. 422-426. (2013)

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Abstract

The objective of this work was to collect isolates of the white rot fungus Ganoderma from decaying woods and investigate their laccase producing and detoxification abilities against aniline and phenol compounds. Sporocarps were collected and the tissue culturing technique was used for fungal isolation. In these experiments specific medium, containing benomyl, dichloran and guaiacol was used. Laccase producing of the isolates were tested in liquid media containing different inducers. Crude enzyme extracts were prepared and characterized. The best laccase-producing strains were identified with partial ITS sequence analysis. Their secreted laccases were investigated with SDS-PAGE and the molecular weights of these enzymes were estimated. Degradation of 7 different aniline and phenol derivates (2,4-dichlorophenol, 2-methyl-4- chlorophenol, 3-chloroaniline, 4-chloroaniline, 2,6-dimethylaniline, 3,4-dichloroaniline and 3-chloro-4- methylaniline) were investigated, observing high degradation rates in most cases.

Item Type: Article
Journal or Publication Title: Review on agriculture and rural development
Date: 2013
Volume: 2
Number: 1
Page Range: pp. 422-426
ISSN: 2063-4803
Uncontrolled Keywords: Mikrobiológia, Analitikai kémia - enzimek, Bioinformatika
Additional Information: Bibliogr.: p. 425-426. ; Összefoglalás angol nyelven
Date Deposited: 2018. Jun. 17. 12:55
Last Modified: 2018. Jul. 26. 11:16
URI: http://acta.bibl.u-szeged.hu/id/eprint/55293

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