Mohammed El-Sayed El-Mahrouk and Dewir Yaser Hassan and Singh Nisha: Indirect shoot organogenesis and plantlets regeneration from stem of ornamental Dieffenbachia maculata cv. Marianna. In: Acta biologica Szegediensis, (51) 2. pp. 113-116. (2007)
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Abstract
The present study reports a simple protocol for indirect shoot organogenesis and plantlets regeneration of Dieffenbachia maculata cv. Marianna using stem segments from in vitro shoot culture. Different concentrations and combinations of indole-3-acetic acid (IAA), 1- naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2, 4-D), 6-benzyladenine (BA), and kinetin (Kin) were used for callus induction and shoot organogenesis. The frequency of callus formation reached 87% and the highest number of shoots per callus was 4.8 for explants cultured on Murashige and Skoog (1962) medium supplemented with 15 mg I'1 BA + 15 mg I'1 NAA. Shoots were further grown and rooted on MS hormone free medium. Regenerated plantlets were successfully acclimatized in greenhouse with 100% survival rate.
Item Type: | Article |
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Heading title: | Article |
Journal or Publication Title: | Acta biologica Szegediensis |
Date: | 2007 |
Volume: | 51 |
Number: | 2 |
ISSN: | 1588-385X |
Page Range: | pp. 113-116 |
Related URLs: | http://acta.bibl.u-szeged.hu/55383/ |
Uncontrolled Keywords: | Akklimatizálódás - kontyvirágfélék |
Additional Information: | Bibliogr.: 116. p., illusztrációkkal |
Date Deposited: | 2018. May. 30. 12:09 |
Last Modified: | 2021. Apr. 13. 10:13 |
URI: | http://acta.bibl.u-szeged.hu/id/eprint/55385 |
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