Indirect shoot organogenesis and plantlets regeneration from stem of ornamental Dieffenbachia maculata cv. Marianna

Mohammed, El-Sayed El-Mahrouk and Dewir, Yaser Hassan and Singh, Nisha: Indirect shoot organogenesis and plantlets regeneration from stem of ornamental Dieffenbachia maculata cv. Marianna. Acta biologica Szegediensis, (51) 2. pp. 113-116. (2007)

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Abstract

The present study reports a simple protocol for indirect shoot organogenesis and plantlets regeneration of Dieffenbachia maculata cv. Marianna using stem segments from in vitro shoot culture. Different concentrations and combinations of indole-3-acetic acid (IAA), 1- naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2, 4-D), 6-benzyladenine (BA), and kinetin (Kin) were used for callus induction and shoot organogenesis. The frequency of callus formation reached 87% and the highest number of shoots per callus was 4.8 for explants cultured on Murashige and Skoog (1962) medium supplemented with 15 mg I'1 BA + 15 mg I'1 NAA. Shoots were further grown and rooted on MS hormone free medium. Regenerated plantlets were successfully acclimatized in greenhouse with 100% survival rate.

Item Type: Article
Journal or Publication Title: Acta biologica Szegediensis
Date: 2007
Volume: 51
Number: 2
Page Range: pp. 113-116
ISSN: 1588-385X
Language: angol
Heading title: Article
Uncontrolled Keywords: Akklimatizálódás - kontyvirágfélék
Additional Information: Bibliogr.: 116. p., illusztrációkkal
Date Deposited: 2018. May. 30. 12:09
Last Modified: 2018. May. 30. 12:09
URI: http://acta.bibl.u-szeged.hu/id/eprint/55385

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