Bajtai Attila; Veres Gábor; Szpisjak László; Ilisz István; Klivényi Péter; Vécsei László; Zádori Dénes: Humán szérum tokoferol- és ubikinonszintek bioanalitikai vizsgálata.
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Absztrakt (kivonat)
The vitamin E group of compounds and ubiquinone are widely known substances with antioxidant properties. However, the setup of high-performance liquid chromatography (HPLC) methods for the detection of these molecules in human serum are often difficult. We report here a reversed-phase HPLC method using diode array detection and a single C18 column separation kept at 25 °C with an isocratic system of acetonitrile, tetrahydrofuran, methanol, ammonium acetate and water at a flow-rate of 2.1 mL/min. This method resolves 3 tocopherols (α, γ, δ) and ubiquinone from human serum within 17 minutes with the application of tocol as an internal standard. We conclude that our procedure provides an adequate sample preparation and separation of the 3 examined tocopherols (α, γ, δ). Although the developed method has been proven to be efficient, accurate and inexpensive, further improvements in the quantitative assessment of ubiquinone are necessary.
| Mű típusa: | Konferencia vagy workshop anyag |
|---|---|
| Befoglaló folyóirat/kiadvány címe: | Proceedings of the International Symposium on Analytical and Environmental Problems |
| Dátum: | 2015 |
| Kötet: | 21 |
| ISBN: | 978-963-306-411-5 |
| Oldalak: | pp. 316-319 |
| Konferencia neve: | International Symposium on Analytical and Environmental Problems (21.) (2015) (Szeged) |
| Befoglaló mű URL: | http://acta.bibl.u-szeged.hu/55892/ |
| Kulcsszavak: | Analitikai kémia |
| Megjegyzések: | Bibliogr.: 319. p. ; összefoglalás angol nyelven |
| Feltöltés dátuma: | 2018. dec. 06. 14:47 |
| Utolsó módosítás: | 2022. aug. 08. 15:47 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/55991 |
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