Humán szérum tokoferol- és ubikinonszintek bioanalitikai vizsgálata

Bajtai, Attila and Veres, Gábor and Szpisjak, László and Ilisz, István and Klivényi, Péter and Vécsei, László and Zádori, Dénes: Humán szérum tokoferol- és ubikinonszintek bioanalitikai vizsgálata. Proceedings of the International Symposium on Analytical and Environmental Problems, (21). pp. 316-319. (2015)

[img] Cikk, tanulmány, mű
proceedings_of_isaep_2015_316-319.pdf

Download (680kB)

Abstract

The vitamin E group of compounds and ubiquinone are widely known substances with antioxidant properties. However, the setup of high-performance liquid chromatography (HPLC) methods for the detection of these molecules in human serum are often difficult. We report here a reversed-phase HPLC method using diode array detection and a single C18 column separation kept at 25 °C with an isocratic system of acetonitrile, tetrahydrofuran, methanol, ammonium acetate and water at a flow-rate of 2.1 mL/min. This method resolves 3 tocopherols (α, γ, δ) and ubiquinone from human serum within 17 minutes with the application of tocol as an internal standard. We conclude that our procedure provides an adequate sample preparation and separation of the 3 examined tocopherols (α, γ, δ). Although the developed method has been proven to be efficient, accurate and inexpensive, further improvements in the quantitative assessment of ubiquinone are necessary.

Item Type: Article
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2015
Volume: 21
Page Range: pp. 316-319
ISBN: 978-963-306-411-5
Uncontrolled Keywords: Analitikai kémia
Additional Information: Bibliogr.: 319. p. ; összefoglalás angol nyelven
Date Deposited: 2018. Dec. 06. 14:47
Last Modified: 2018. Dec. 06. 14:47
URI: http://acta.bibl.u-szeged.hu/id/eprint/55991

Actions (login required)

View Item View Item