E-vitamin mérésre alkalmas nagyhatékonyságú folyadékkromatográfiás módszerek fejlesztése és validálása = Development and validation of high performance liquid chromatography methods for vitamin E measurements

Nánási, Nikolett and Cseh, Edina and Szentirmai, Márton and Veres, Gábor and Klivényi, Péter and Vécsei, László and Zádori, Dénes: E-vitamin mérésre alkalmas nagyhatékonyságú folyadékkromatográfiás módszerek fejlesztése és validálása = Development and validation of high performance liquid chromatography methods for vitamin E measurements. In: Proceedings of the International Symposium on Analytical and Environmental Problems, (23). pp. 469-473. (2017)

[img]
Preview
Cikk, tanulmány, mű
proceedings_of_isaep_2017_469-473.pdf

Download (593kB) | Preview

Abstract

Tocopherols, also known as the ingredients of fat-soluble vitamin E compounds play an important role in antioxidant mechanisms. Their deficiency of tocopherols, mainly that of αtocopherol, can cause several neurological disorders, such as ataxia, myopathy, retinopathy, peripheral neuropathy. The aim of this study was to validate and establish methods applicable for the detection of α-tocopherol from murine serum and certain brain regions. In the present study we report 3 different reversed-phase HPLC methods using a single C18 column in isocratic system. The detection was carried out with 3 different detectors. For the detection of murine serum samples, a diode-array detector (DAD) was applied, whereas for murine brain samples electrochemical (ECD) and fluorescence detectors (FLD) were utilized and a detailed validation process was carried out. The ranges of precision and recovery were the following during the validation processes: 0.61-3.57% and 66.19-105.91%, respectively. The application of these methods provide a valuable tool for the determination of αtocopherol from murine serum and brain samples.

Item Type: Article
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2017
Volume: 23
ISBN: 978-963-306-563-1
Page Range: pp. 469-473
Uncontrolled Keywords: Vitamin - E, Idegrendszerre ható anyagok - gyógyszerhatástan, Biokémia
Additional Information: Bibliogr.: 473. p. ; összefoglalás angol nyelven
Date Deposited: 2019. Jan. 03. 10:25
Last Modified: 2019. Jan. 03. 10:25
URI: http://acta.bibl.u-szeged.hu/id/eprint/56251

Actions (login required)

View Item View Item