Selvam Kanesan Panneer and Nambirajan Guruvu and Annamalai Balasubramaniam and Alaidarous Mohammed and Alshehri Bader Mohammed and Bin Dukhyil Abdul Aziz A. and Shobana Coimbatore Subramanian and Manikandan Palanisamy: Expression and production optimization of the cationic antimicrobial peptide : indolicidin by the recombinant E. coli C41 (DE3) clones. In: Acta biologica Szegediensis, (62) 1. pp. 61-66. (2018)
Preview |
Cikk, tanulmány, mű
biologica_062_numb_001_061-066.pdf Download (2MB) | Preview |
Abstract
The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Acta biologica Szegediensis |
| Date: | 2018 |
| Volume: | 62 |
| Number: | 1 |
| ISSN: | 1588-4082 |
| Page Range: | pp. 61-66 |
| Publisher: | University of Szeged |
| Place of Publication: | Szeged |
| Related URLs: | http://acta.bibl.u-szeged.hu/62209/ |
| DOI: | 10.14232/abs.2018.1.61-66 |
| Uncontrolled Keywords: | Mikrobiológia |
| Additional Information: | Bibliogr.: p. 65-66. ; összefoglalás angol nyelven |
| Date Deposited: | 2019. Nov. 21. 14:09 |
| Last Modified: | 2021. Apr. 12. 14:06 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/58199 |
Actions (login required)
 |
View Item |
