Expression and production optimization of the cationic antimicrobial peptide : indolicidin by the recombinant E. coli C41 (DE3) clones

Selvam, Kanesan Panneer and Nambirajan, Guruvu and Annamalai, Balasubramaniam and Alaidarous, Mohammed and Alshehri, Bader Mohammed and Bin Dukhyil, Abdul Aziz A. and Shobana, Coimbatore Subramanian and Manikandan, Palanisamy: Expression and production optimization of the cationic antimicrobial peptide : indolicidin by the recombinant E. coli C41 (DE3) clones. In: Acta biologica Szegediensis, (62) 1. pp. 61-66. (2018)

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Abstract

The cytoplasmic granules of bovine neutrophils naturally possess indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask level production and expression optimizations of the indolicidin by the recombinant Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions required for maximal production. It was determined that a concentration of 1 mM of IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation were required for maximal indolicidin expression.

Item Type: Article
Journal or Publication Title: Acta biologica Szegediensis
Date: 2018
Volume: 62
Number: 1
ISSN: 1588-4082
Page Range: pp. 61-66
Publisher: University of Szeged
Place of Publication: Szeged
DOI: https://doi.org/10.14232/abs.2018.1.61-66
Uncontrolled Keywords: Mikrobiológia
Additional Information: Bibliogr.: p. 65-66. ; összefoglalás angol nyelven
Date Deposited: 2019. Nov. 21. 14:09
Last Modified: 2019. Nov. 21. 14:09
URI: http://acta.bibl.u-szeged.hu/id/eprint/58199

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