Veres Gábor; Tellér Andrea; Martos Diána; Szatmári István; Kiss Lóránd; Vécsei László; Zádori Dénes: Determination of glutamate and gaba from rat central nervous system samples with HPLC utilizing fluorescent detection.
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Absztrakt (kivonat)
The research of neurological diseases has great importance and the determination of neurotransmitter concentrations is essential during these investigations. The levels of main excitatory (glutamate) and inhibitory (γ-amino-butyric acid (GABA)) neurotransmitters often change as a result of pathological alterations. For example, in case of migraine, elevated glutamate release is suggested to be a part of the pathomechanism, causing hypersensitivity to pain. Our goal in this study was to optimize a liquid chromatography method to measure glutamate and GABA from the trigeminal nucleus pars caudalis (TNC) of rats, which is responsible for pain processing. As a result, we were able to validate our method according to international guidelines where the investigated parameters were LOD, LOQ, precision and recovery. Furthermore, we applied a new internal standard which has not been published so far. This method will be utilized in the investigation of migraine animal models to evaluate potential new therapeutic approaches.
| Mű típusa: | Konferencia vagy workshop anyag |
|---|---|
| Befoglaló folyóirat/kiadvány címe: | Proceedings of the International Symposium on Analytical and Environmental Problems |
| Dátum: | 2019 |
| ISBN: | 978-963-306-702-4 |
| Oldalak: | pp. 427-431 |
| Konferencia neve: | International Symposium on Analytical and Environmental Problems (25.) (2019) (Szeged) |
| Befoglaló mű URL: | http://acta.bibl.u-szeged.hu/64947/ |
| Kulcsszavak: | Ideggyógyászat - kutatás, Gyógyszerkémia |
| Megjegyzések: | Bibliogr.: 431. p. ; összefoglalás angol nyelven |
| Feltöltés dátuma: | 2020. már. 23. 11:07 |
| Utolsó módosítás: | 2022. aug. 08. 15:37 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/64899 |
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