Determination of glutamate and gaba from rat central nervous system samples with HPLC utilizing fluorescent detection

Veres Gábor and Tellér Andrea and Martos Diána and Szatmári István and Kiss Lóránd and Vécsei László and Zádori Dénes: Determination of glutamate and gaba from rat central nervous system samples with HPLC utilizing fluorescent detection.

[thumbnail of proceedings_of_isaep_2019_427-431.pdf]
Preview
Cikk, tanulmány, mű
proceedings_of_isaep_2019_427-431.pdf

Download (772kB) | Preview

Abstract

The research of neurological diseases has great importance and the determination of neurotransmitter concentrations is essential during these investigations. The levels of main excitatory (glutamate) and inhibitory (γ-amino-butyric acid (GABA)) neurotransmitters often change as a result of pathological alterations. For example, in case of migraine, elevated glutamate release is suggested to be a part of the pathomechanism, causing hypersensitivity to pain. Our goal in this study was to optimize a liquid chromatography method to measure glutamate and GABA from the trigeminal nucleus pars caudalis (TNC) of rats, which is responsible for pain processing. As a result, we were able to validate our method according to international guidelines where the investigated parameters were LOD, LOQ, precision and recovery. Furthermore, we applied a new internal standard which has not been published so far. This method will be utilized in the investigation of migraine animal models to evaluate potential new therapeutic approaches.

Item Type: Conference or Workshop Item
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2019
ISBN: 978-963-306-702-4
Page Range: pp. 427-431
Event Title: International Symposium on Analytical and Environmental Problems (25.) (2019) (Szeged)
Related URLs: http://acta.bibl.u-szeged.hu/64947/
Uncontrolled Keywords: Ideggyógyászat - kutatás, Gyógyszerkémia
Additional Information: Bibliogr.: 431. p. ; összefoglalás angol nyelven
Date Deposited: 2020. Mar. 23. 11:07
Last Modified: 2022. Aug. 08. 15:37
URI: http://acta.bibl.u-szeged.hu/id/eprint/64899

Actions (login required)

View Item View Item