Halder Suman K.; Mondal Joy; Hor Papan K.; Mohapatra Pradeep K. D.; Mondal Keshab C.; Soren Jyoti Prakash: A permissive approach for optimization of L-glutaminase production using wheat bran as supporting substrate and assessment of its cytotoxic potentialities. In: Acta biologica Szegediensis, (64) 1. pp. 1-10. (2020)
Előnézet |
Cikk, tanulmány, mű
biologica_064_numb_001_001-010.pdf Letöltés (3MB) | Előnézet |
Absztrakt (kivonat)
Microbial L-glutaminase has considered as one of the most important therapeutic enzymes considering its anticancer or antitumor activity. In this study, one L-glutaminase producing potent fungus was isolated from the coastal soil and identified as Fusarium nelsonii KPJ-2. During parametric optimization, it was noted that wheat bran supported maximum L-glutaminase production than other agro-industrial wastes tested. Solid substrate fermentation was mechanized with optimum pH of 4.0, incubation temperature at 25 °C, inoculums concentration of 2.0% (v/v), substrate concentration of 7.0% (w/v) and moisture of the production media suits at 20.0% (w/v). Statistical optimization using Response Surface Methodology (RSM) was improved the L-glutaminase production by 14.5% (68.93 U/gds) than unoptimized state. The SEM-EDX analysis demonstrated the overgrowth of fungus on wheat bran and utilization of its associated minerals. A comparative cytotoxic effect of the partial purified glutaminase was examined on both cancerous HCT cell and normal Vero cell line. The result clearly demonstrated that L-glutaminase from F. nelsonii KPJ-2 is specifically cytotoxic against cancer cell line with IC50 of 203.95µg/ml, but, non-responsive against normal cell. The newly isolated fungal strain can produce a considerable amount of L-glutaminase utilizing very low-cost substrate and the enzyme have therapeutic value for real life application owing to its anticancer effectiveness.
| Mű típusa: | Cikk, tanulmány, mű |
|---|---|
| Befoglaló folyóirat/kiadvány címe: | Acta biologica Szegediensis |
| Dátum: | 2020 |
| Kötet: | 64 |
| Szám: | 1 |
| ISSN: | 1588-385X |
| Oldalak: | pp. 1-10 |
| Nyelv: | angol |
| Kiadó: | University of Szeged |
| Kiadás helye: | Szeged |
| Befoglaló mű URL: | http://acta.bibl.u-szeged.hu/71095/ |
| DOI: | 10.14232/abs.2020.1.1-10 |
| Kulcsszavak: | Mikrobiológia |
| Megjegyzések: | Bibliogr.: p. 9-10. ; ill. ; összefoglalás angol nyelven |
| Szakterület: | 01. Természettudományok 01. Természettudományok > 01.06. Biológiai tudományok |
| Feltöltés dátuma: | 2020. dec. 07. 13:25 |
| Utolsó módosítás: | 2023. feb. 22. 14:03 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/70956 |
 |
Tétel nézet |
