A permissive approach for optimization of L-glutaminase production using wheat bran as supporting substrate and assessment of its cytotoxic potentialities

Halder Suman K.; Mondal Joy; Hor Papan K.; Mohapatra Pradeep K. D.; Mondal Keshab C.; Soren Jyoti Prakash: A permissive approach for optimization of L-glutaminase production using wheat bran as supporting substrate and assessment of its cytotoxic potentialities. In: Acta biologica Szegediensis, (64) 1. pp. 1-10. (2020)

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Microbial L-glutaminase has considered as one of the most important therapeutic enzymes considering its anticancer or antitumor activity. In this study, one L-glutaminase producing potent fungus was isolated from the coastal soil and identified as Fusarium nelsonii KPJ-2. During parametric optimization, it was noted that wheat bran supported maximum L-glutaminase production than other agro-industrial wastes tested. Solid substrate fermentation was mechanized with optimum pH of 4.0, incubation temperature at 25 °C, inoculums concentration of 2.0% (v/v), substrate concentration of 7.0% (w/v) and moisture of the production media suits at 20.0% (w/v). Statistical optimization using Response Surface Methodology (RSM) was improved the L-glutaminase production by 14.5% (68.93 U/gds) than unoptimized state. The SEM-EDX analysis demonstrated the overgrowth of fungus on wheat bran and utilization of its associated minerals. A comparative cytotoxic effect of the partial purified glutaminase was examined on both cancerous HCT cell and normal Vero cell line. The result clearly demonstrated that L-glutaminase from F. nelsonii KPJ-2 is specifically cytotoxic against cancer cell line with IC50 of 203.95µg/ml, but, non-responsive against normal cell. The newly isolated fungal strain can produce a considerable amount of L-glutaminase utilizing very low-cost substrate and the enzyme have therapeutic value for real life application owing to its anticancer effectiveness.

Mű típusa: Cikk, tanulmány, mű
Befoglaló folyóirat/kiadvány címe: Acta biologica Szegediensis
Dátum: 2020
Kötet: 64
Szám: 1
ISSN: 1588-385X
Oldalak: pp. 1-10
Nyelv: angol
Kiadó: University of Szeged
Kiadás helye: Szeged
Befoglaló mű URL: http://acta.bibl.u-szeged.hu/71095/
DOI: 10.14232/abs.2020.1.1-10
Kulcsszavak: Mikrobiológia
Megjegyzések: Bibliogr.: p. 9-10. ; ill. ; összefoglalás angol nyelven
Szakterület: 01. Természettudományok
01. Természettudományok > 01.06. Biológiai tudományok
Feltöltés dátuma: 2020. dec. 07. 13:25
Utolsó módosítás: 2023. feb. 22. 14:03
URI: http://acta.bibl.u-szeged.hu/id/eprint/70956
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