A permissive approach for optimization of L-glutaminase production using wheat bran as supporting substrate and assessment of its cytotoxic potentialities

Halder Suman K. and Mondal Joy and Hor Papan K. and Mohapatra Pradeep K. D. and Mondal Keshab C. and Soren Jyoti Prakash: A permissive approach for optimization of L-glutaminase production using wheat bran as supporting substrate and assessment of its cytotoxic potentialities. In: Acta biologica Szegediensis, (64) 1. pp. 1-10. (2020)

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Abstract

Microbial L-glutaminase has considered as one of the most important therapeutic enzymes considering its anticancer or antitumor activity. In this study, one L-glutaminase producing potent fungus was isolated from the coastal soil and identified as Fusarium nelsonii KPJ-2. During parametric optimization, it was noted that wheat bran supported maximum L-glutaminase production than other agro-industrial wastes tested. Solid substrate fermentation was mechanized with optimum pH of 4.0, incubation temperature at 25 °C, inoculums concentration of 2.0% (v/v), substrate concentration of 7.0% (w/v) and moisture of the production media suits at 20.0% (w/v). Statistical optimization using Response Surface Methodology (RSM) was improved the L-glutaminase production by 14.5% (68.93 U/gds) than unoptimized state. The SEM-EDX analysis demonstrated the overgrowth of fungus on wheat bran and utilization of its associated minerals. A comparative cytotoxic effect of the partial purified glutaminase was examined on both cancerous HCT cell and normal Vero cell line. The result clearly demonstrated that L-glutaminase from F. nelsonii KPJ-2 is specifically cytotoxic against cancer cell line with IC50 of 203.95µg/ml, but, non-responsive against normal cell. The newly isolated fungal strain can produce a considerable amount of L-glutaminase utilizing very low-cost substrate and the enzyme have therapeutic value for real life application owing to its anticancer effectiveness.

Item Type: Article
Journal or Publication Title: Acta biologica Szegediensis
Date: 2020
Volume: 64
Number: 1
ISSN: 1588-385X
Page Range: pp. 1-10
Publisher: University of Szeged
Place of Publication: Szeged
Related URLs: http://acta.bibl.u-szeged.hu/71095/
DOI: 10.14232/abs.2020.1.1-10
Uncontrolled Keywords: Mikrobiológia
Additional Information: Bibliogr.: p. 9-10. ; ill. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.06. Biological sciences
01. Natural sciences > 01.06. Biological sciences > 01.06.01. Cell biology, microbiology
01. Natural sciences > 01.06. Biological sciences > 01.06.01. Cell biology, microbiology > 01.06.01.01. Microbiology
Date Deposited: 2020. Dec. 07. 13:25
Last Modified: 2020. Dec. 07. 13:25
URI: http://acta.bibl.u-szeged.hu/id/eprint/70956

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