Waste-wood derived biochar as a support for horseradish peroxidase immobilization

Petronijević Mirjana and Panić Sanja and Đurović-Mladenović Nataša and Savić Saša: Waste-wood derived biochar as a support for horseradish peroxidase immobilization.

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Abstract

In this paper, the suitability of waste-wood derived biochar particles as a support for the horseradish peroxidase (HRP) immobilization by adsorption method was investigated. The change in enzymatic activity of the immobilized enzyme at different values of pH and temperature, as well as stability over time, was measured. The results showed that HRP can efficiently bind to biochar particles by adsorption. The immobilized enzyme shows high activity (>80%) at a wide range of pH (7-9) and temperature (20-50°C). The immobilized enzyme retains 22% and 40% of its activity during storage at temperatures of 25 and 10°C after a period of 30 days, respectively.

Item Type: Conference or Workshop Item
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2020
Volume: 26
ISBN: 978-963-306-771-0
Page Range: pp. 286-288
Language: English
Event Title: International Symposium on Analytical and Environmental Problems (26.) (2020) (Szeged)
Related URLs: http://acta.bibl.u-szeged.hu/73792/
Uncontrolled Keywords: Analitikai kémia
Additional Information: Bibliogr.: 288. p. ; ill. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.04. Chemical sciences
Date Deposited: 2021. Nov. 18. 09:48
Last Modified: 2022. Aug. 08. 15:39
URI: http://acta.bibl.u-szeged.hu/id/eprint/74016

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