Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe dffusa and embryotoxicity assessment in a chicken embryo model

Cruz Mycheli P. da and Larentis Lucas T. and Vismara Edgar de S. and Vismara Lilian de S. and Freitas Patricia F. de and Mazaro Sérgio M.: Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe dffusa and embryotoxicity assessment in a chicken embryo model. In: Acta biologica Szegediensis, (65) 1. pp. 47-57. (2021)

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Abstract

This work aimed to evaluate the antimicrobial effect of Ganoderma lucidum mycelial growth filtrates (MGF) on the phytopathogen Erysiphe diffusa and their potential effects on the embryonic development of Gallus gallus. The antimicrobial activity was evaluated on E. diffusa spores by the microdilution broth method. To evaluate embryotoxic and teratogenic effects, fertile eggs of G. gallus received injections of solutions containing the filtrates of G. lucidum through the air chamber. After three days of incubation, we opened the eggs and evaluated egg viability, embryo survival, malformation occurrence, embryonic staging and heart rate. Live embryos were prepared using whole mount technique and the morphological analysis was performed. We used the generalized linear model to fit embryotoxicity and teratogenicity data. We verified that G. lucidum MGF showed inhibitory activity in vitro against E. diffusa and the minimum inhibitory concentrations ranged from 5 to 10 mg/mL. We could also observe that the filtrates did not present embryotoxic or teratogenic effects on the early embryonic development of G. gallus, but induced significant differences in the embryonic mean heart rate and on the stage of embryonic development.

Item Type:	Article
Journal or Publication Title:	Acta biologica Szegediensis
Date:	2021
Volume:	65
Number:	1
ISSN:	1588-4082
Page Range:	pp. 47-57
Language:	English
Publisher:	University of Szeged
Place of Publication:	Szeged
Related URLs:	http://acta.bibl.u-szeged.hu/75563/
DOI:	10.14232/abs.2021.1.47-57
Uncontrolled Keywords:	Laboratóriumi technika - biológia
Additional Information:	Bibliogr.: p. 54-57. ; ill. ; összefoglalás angol nyelven
Subjects:	01. Natural sciences 01. Natural sciences > 01.06. Biological sciences
Date Deposited:	2022. May. 12. 08:40
Last Modified:	2022. May. 12. 08:40
URI:	http://acta.bibl.u-szeged.hu/id/eprint/75579

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