Perturbation of the mucosa-associated anaerobic gut microbiota in streptozotocin-induced diabetic rats

Wirth Roland and Bódi Nikolett and Szalai Zita and Chandrakumar Lalitha and Maróti Gergely and Kovács Kornél L and Bagi Zoltán and Mezei Diána and Balázs János and Bagyánszki Mária: Perturbation of the mucosa-associated anaerobic gut microbiota in streptozotocin-induced diabetic rats. In: Acta biologica Szegediensis, (65) 1. pp. 75-84. (2021)

[thumbnail of biologica_065_numb_001_075-084.pdf] Cikk, tanulmány, mű

Download (3MB)


Our aim was to map the gut region-specific differences of the mucosa-associated microbiome distribution in a streptozotocin-induced diabetic rat model. Tissue samples from the duodenum, ileum and colon were collected 10 weeks after the onset of hyperglycaemia to analyse the mucosa-associated microbiota using next-generation DNA sequencing. Striking differences were observed in the mucosa-associated microbiota of the duodenum between diabetic and control rats. A significant invasion of the aerobic genus Mycoplasma was apparent in diabetes, and the abundance of the anaerobic phylum Firmicutes decreased massively. It is noteworthy that insulin treatment eliminated the Mycoplasma invasion in the duodenum and apparently restored the anaerobic environment in the mucosa. In the ileum the abundance of the phylum Firmicutes increased in the diabetic samples. Although the proportion of the phylum Proteobacteria decreased moderately, its composition changed significantly, and insulin treatment induced only minor alterations. In the diabetic samples of colon, the abundance of the phylum Firmicutes decreased slightly, the relative number of the bacteria in the phylum Bacteroidetes increased strongly as compared to the control values, and after insulin treatment this increase was more significant. Chronic hyperglycaemia has the most prominent effect on the mucosa-associated microbiota in the duodenum.

Item Type: Article
Journal or Publication Title: Acta biologica Szegediensis
Date: 2021
Volume: 65
Number: 1
ISSN: 1588-4082
Page Range: pp. 75-84
Language: English
Publisher: University of Szeged
Place of Publication: Szeged
Related URLs:
DOI: 10.14232/abs.2021.1.75-84
Uncontrolled Keywords: Állatkísérletek, Kísérlet - biológiai, Cukorbetegség
Additional Information: Bibliogr.: p. 82-84. ; ill. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.06. Biological sciences
Date Deposited: 2022. May. 12. 09:23
Last Modified: 2022. May. 12. 09:23

Actions (login required)

View Item View Item