Gajdács Márió; Kárpáti Krisztina; Stájer Anette; Zanetti Stefania; Donadu Matthew Gavino: Insights on carbapenem-resistant Pseudomonas aeruginosa : phenotypic characterization of relevant isolates. In: Acta biologica Szegediensis, (65) 1. pp. 105-112. (2021)
![[thumbnail of biologica_065_numb_001_105-112.pdf]](http://acta.bibl.u-szeged.hu/style/images/fileicons/part.png) |
Cikk, tanulmány, mű
biologica_065_numb_001_105-112.pdf Letöltés (1MB) |
Absztrakt (kivonat)
Pseudomonas aeruginosa (P. aeruginosa) is ubiquitous in nature, and may be a causative agent in severe, life-threatening infections. In >60% of cases, β-lactam antibiotics are used in the therapy of P. aeruginosa infections, therefore the emergence of carbapenem-resistant P. aeruginosa (CRPA) is a significant clinical concern. In this study, phenotypic methods were used to characterize fifty-four (n = 54) P. aeruginosa isolates, which were included based on their suspected non-susceptibility to meropenem. Minimum inhibitory concentrations (MICs) of meropenem, ceftazidime, cefepime, ciprofloxacin, gentamicin, were determined using E-tests, while colistin MICs were determined using broth microdilution. The isolates were subjected to the modified Hodge test (MHT), the modified carbapenem-inactivation method (mCIM) and the imipenem/EDTA combined disk test (CDT). AmpC and efflux pump overexpression was studied using agar plates containing cloxacillin and phenylalanine-arginine β-naphthylamide (PAβN), respectively. Assessment of biofilm-formation was carried out using the crystal violet tube-adherence method. 38.9% of the strains showed meropenem MICs in the resistant range (>8 mg/L). Efflux-pump overexpression and AmpC-hyperproduction was seen in 44.4% and 35.2% of isolates, respectively. 88.8% of the isolates were characterized as strong biofilm-producers. On the other hand, the presence of carbapenemases was suspected in a minority (16.7%) of tested isolates. As safe and effective therapeutic options in carbapenem-resistant Gram-negative infections are severely limited, characterization of these isolates using phenotypic and molecular-based methods is important to provide insights into the epidemiological features of these pathogens.
| Mű típusa: | Cikk, tanulmány, mű |
|---|---|
| Befoglaló folyóirat/kiadvány címe: | Acta biologica Szegediensis |
| Dátum: | 2021 |
| Kötet: | 65 |
| Szám: | 1 |
| ISSN: | 1588-4082 |
| Oldalak: | pp. 105-112 |
| Nyelv: | angol |
| Kiadó: | University of Szeged |
| Kiadás helye: | Szeged |
| Befoglaló mű URL: | http://acta.bibl.u-szeged.hu/75563/ |
| DOI: | 10.14232/abs.2021.1.105-112 |
| Kulcsszavak: | Mikrobiológia |
| Megjegyzések: | Bibliogr.: p. 110-112. ; összefoglalás angol nyelven |
| Szakterület: | 01. Természettudományok 01. Természettudományok > 01.06. Biológiai tudományok |
| Feltöltés dátuma: | 2022. máj. 12. 09:59 |
| Utolsó módosítás: | 2022. máj. 12. 09:59 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/75585 |
 |
Tétel nézet |
