Accurate detection of SARS-CoV-2 might be a challenge in the molecular biology laboratory for RT-PCR final results

Ahmadi Mirela and Oarga Doris and Bica Valerica and Ilianu Delis Rinaldo and Chende Dragoș and Peț Ioan and Ştef Lavinia and Radulov Isidora and Iancu Tiberiu and Acatincăi Stelian and Dronca Dorel: Accurate detection of SARS-CoV-2 might be a challenge in the molecular biology laboratory for RT-PCR final results.

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Abstract

The challenges we experience professionally always teach us to retreat, to document ourselves, to learn, to become better and to succeed in asserting ourselves in the fields we have trained and perfected throughout several years. This also happened in the pandemic times with Covid19, when we had to document RT-PCR techniques in order to be able to detect at the molecular level the SARS-CoV-2 virus responsible for disturbing the world worldwide. Thus, in the molecular biology laboratory, analysts must make very important decisions about the final result of the RT-PCR test to determine – given several analytical criteria – whether the result is negative, positive or equivocal. There are situations where the RT-PCR equipment does not detect all the genes responsible for a clearly positive result, or when the cycle threshold (Ct) is higher than specified in the reagent insert kit, and then it is the analyst's duty to decide what is the final result of molecular test. This paper brings to the fore the involvement, duty and the art of researchers and specialists who must assume the final result of the RT-PCR test in the detection of the SARS-CoV-2 virus responsible for the global pandemic of Covid-19.

Item Type:	Conference or Workshop Item
Journal or Publication Title:	Proceedings of the International Symposium on Analytical and Environmental Problems
Date:	2021
Volume:	27
ISBN:	978-963-306-835-9
Page Range:	pp. 72-76
Language:	English
Publisher:	University of Szeged
Place of Publication:	Szeged
Event Title:	International Symposium on Analytical and Environmental Problems (27.) (2021) (Szeged)
Related URLs:	http://acta.bibl.u-szeged.hu/75798/
Uncontrolled Keywords:	Biológia - molekuláris
Additional Information:	Bibliogr.: 76. p. ; összefoglalás angol nyelven
Subjects:	01. Natural sciences 01. Natural sciences > 01.06. Biological sciences
Date Deposited:	2022. May. 26. 12:53
Last Modified:	2022. Aug. 08. 15:40
URI:	http://acta.bibl.u-szeged.hu/id/eprint/75926

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