In vitro mutagenicity evaluation of iron oxide nanoparticles by the bacterial reverse mutation assay

Szalay Brigitta and Vezér Tünde: In vitro mutagenicity evaluation of iron oxide nanoparticles by the bacterial reverse mutation assay.

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Abstract

Iron oxide nanoparticles are becoming highly promising tools for a wide spectrum of biomedical applications. The aim of our work was to determine the potential mutagenic effects of Fe2C>3 and FesCU nanoparticles (NPs), using bacterial reverse mutation assay. This is still the most widely used method for evaluating chemicals and environmental samples for mutagenic activity. In the bacterial reverse mutation assay, two genotypic variants of the Salmonella typhimurium strains (TA98 and TA1535) were used. Fe2Ü3 and Fe3Ü4 NPs were incubated with these two strains in four different doses both in presence and in absence of the rat liver metabolic activation system (S9); concurrently, appropriate positive controls were used to validate the test. The assessment of the results was based on the number of reverse mutants. The average number of reverse mutants per plate treated with NPs was less than double compared to negative control. Fe203 and Fe3C>4 NPs proved to have no mutagenic effect in the bacterial cellular systems tested, in that they did not significantly increase the number of revertant colonies. Our findings indicate that Fe203 and Fe304 NPs are non mutagenic in bacterial reverse mutation assay under the present test conditions. These results are useful to expand our knowledge on the safety of iron oxides NPs.

Item Type:	Conference or Workshop Item
Journal or Publication Title:	Proceedings of the International Symposium on Analytical and Environmental Problems
Date:	2012
Volume:	17
ISBN:	978-963-315-066-5
Page Range:	pp. 275-278
Language:	English
Publisher:	SZAB
Place of Publication:	Szeged
Event Title:	International Symposium on Analytical and Environmental Problems (17.) (2011) (Szeged)
Event Type:	Conference
Event Location:	Szeged
Event Dates:	2011. szeptember 19.
Related URLs:	http://acta.bibl.u-szeged.hu/76381/
Uncontrolled Keywords:	Orvosbiológia
Additional Information:	Bibliogr.: 278. p. ; ill. ; összefoglalás angol nyelven
Subjects:	03. Medical and health sciences 03. Medical and health sciences > 03.04. Medical biotechnology
Date Deposited:	2022. Aug. 10. 11:20
Last Modified:	2022. Aug. 22. 10:23
URI:	http://acta.bibl.u-szeged.hu/id/eprint/76442

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