Gémes Borbála; Takács Eszter; Székács Inna; Farkas Enikő; Horváth Róbert; Klátyik Szandra; Székács András: A newly identified specific biological activity of glyphosate - inhibition of RGD-binding integrins.
Előnézet |
Cikk, tanulmány, mű
proceedings_of_isaep_2022_147-151.pdf Letöltés (627kB) | Előnézet |
Absztrakt (kivonat)
In this study we investigated the inhibitory effect of the widely used broad-spectrum herbicide active ingredient glyphosate and its related analogues on αVβ3 integrin binding to the shortest oligopeptide recognizing motif of integrins, the arginine-glycine-aspartic acid (RGD) sequence. Integrin binding characteristics were assessed in a modified enzyme linked immunosorbent assay (ELISA) and by a label-free optical biosensor technique. At 22 mM, glyphosate reached full inhibition of αVβ3, and the inhibitory activity of its main metabolite, aminomethylphosphonic acid (AMPA) was also above 95%, while another environmentally relevant metabolite, sarcosine exerted only a weaker effect, approximately 35% inhibition. In turn, the half maximal inhibitory concentration (IC50) of glyphosate and AMPA were reported to be 2.7±0.5 mM and 1.3±0.2 mM, respectively. The inhibitory effects of the other related compounds investigated (acetylglycine, glycine and iminodiacetic acid) at the same concentration, 22 mM were below 50%. Inhibitory effects on cell adhesion to RGD-modified surfaces by whole cells containing several types of RGD-binding integrins including αVβ3 were detected using the biosensor technique, where the integrin antagonist activity of glyphosate was also demonstrated.
| Mű típusa: | Konferencia vagy workshop anyag |
|---|---|
| Befoglaló folyóirat/kiadvány címe: | Proceedings of the International Symposium on Analytical and Environmental Problems |
| Dátum: | 2022 |
| Kötet: | 28 |
| ISBN: | 978-963-306-904-2 |
| Oldalak: | pp. 147-151 |
| Nyelv: | angol |
| Kiadás helye: | Szeged |
| Konferencia neve: | 28th International Symposium on Analytical and Environmental Problems |
| Konferencia típusa: | Konferencia |
| Helyszin: | Szeged |
| Dátum: | 2022. november 14-15. |
| Befoglaló mű URL: | https://acta.bibl.u-szeged.hu/78308/ |
| Kulcsszavak: | Kémia |
| Megjegyzések: | Bibliogr.: 151. p. ; ill. ; összefoglalás angol nyelven |
| Szakterület: | 01. Természettudományok 01. Természettudományok > 01.04. Kémiai tudományok |
| Feltöltés dátuma: | 2023. már. 20. 13:04 |
| Utolsó módosítás: | 2023. már. 20. 13:04 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/78491 |
 |
Tétel nézet |
