A newly identified specific biological activity of glyphosate - inhibition of RGD-binding integrins

Gémes Borbála and Takács Eszter and Székács Inna and Farkas Enikő and Horváth Róbert and Klátyik Szandra and Székács András: A newly identified specific biological activity of glyphosate - inhibition of RGD-binding integrins.

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Abstract

In this study we investigated the inhibitory effect of the widely used broad-spectrum herbicide active ingredient glyphosate and its related analogues on αVβ3 integrin binding to the shortest oligopeptide recognizing motif of integrins, the arginine-glycine-aspartic acid (RGD) sequence. Integrin binding characteristics were assessed in a modified enzyme linked immunosorbent assay (ELISA) and by a label-free optical biosensor technique. At 22 mM, glyphosate reached full inhibition of αVβ3, and the inhibitory activity of its main metabolite, aminomethylphosphonic acid (AMPA) was also above 95%, while another environmentally relevant metabolite, sarcosine exerted only a weaker effect, approximately 35% inhibition. In turn, the half maximal inhibitory concentration (IC50) of glyphosate and AMPA were reported to be 2.7±0.5 mM and 1.3±0.2 mM, respectively. The inhibitory effects of the other related compounds investigated (acetylglycine, glycine and iminodiacetic acid) at the same concentration, 22 mM were below 50%. Inhibitory effects on cell adhesion to RGD-modified surfaces by whole cells containing several types of RGD-binding integrins including αVβ3 were detected using the biosensor technique, where the integrin antagonist activity of glyphosate was also demonstrated.

Item Type: Conference or Workshop Item
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2022
Volume: 28
ISBN: 978-963-306-904-2
Page Range: pp. 147-151
Language: English
Place of Publication: Szeged
Event Title: 28th International Symposium on Analytical and Environmental Problems
Event Type: Conference
Event Location: Szeged
Event Dates: 2022. november 14-15.
Related URLs: https://acta.bibl.u-szeged.hu/78308/
Uncontrolled Keywords: Kémia
Additional Information: Bibliogr.: 151. p. ; ill. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.04. Chemical sciences
Date Deposited: 2023. Mar. 20. 13:04
Last Modified: 2023. Mar. 20. 13:04
URI: http://acta.bibl.u-szeged.hu/id/eprint/78491

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