Detailed cytotoxicity assessment of the formulated herbicide roundup classic and its constituents

Oláh Marianna and Farkas Enikő and Székács Inna and Horváth Róbert and Klátyik Szandra and Székács András: Detailed cytotoxicity assessment of the formulated herbicide roundup classic and its constituents.

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Cytotoxicity of the globally market-leading herbicide ROUNDUP CLASSIC formulation and its components such as the active ingredient glyphosate and the formulating agent POEA (a mixture of polyethoxylated tallow amines) were investigated on the murine neuroectodermal stem cell-like (NE-4C) and osteoblastic (MC3T3-E1) cell lines. The cytotoxic and genotoxic effects on cell viability and cell cycles were evaluated based on the results of flow cytometry, enzymatic-assays, and alkaline single cell gel electrophoresis (Comet) assays, furthermore, the effects on cell morphology and dynamic mass redistribution of cellular contents were assessed with the use of the label-free Epic BenchTop optical biosensor on MC3T3-E1 cells adhered on the surface of the biosensor. Differences in the sensitivity of the investigated cell lines were detected, while the MC3T3-E1 cell line indicated less sensitivity to the effects of the treatments. Furthermore, differences were also observed in the sensitivity of the performed assays. The order of the inhibitory potency of the investigated compounds was as follows: glyphosate IPA salt << ROUNDUP CLASSIC < POEA. The applied Epic technique provides an effective tool for the real-time detection of cytotoxicity.

Item Type: Conference or Workshop Item
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2022
Volume: 28
ISBN: 978-963-306-904-2
Page Range: pp. 269-273
Language: English
Place of Publication: Szeged
Event Title: 28th International Symposium on Analytical and Environmental Problems
Event Type: Conference
Event Location: Szeged
Event Dates: 2022. november 14-15.
Related URLs:
Uncontrolled Keywords: Agrokémia, Környezetkémia
Additional Information: Bibliogr.: 273. p. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.04. Chemical sciences
Date Deposited: 2023. Mar. 23. 09:57
Last Modified: 2023. Mar. 23. 09:57

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