Enzyme-linked fluorescent immunoassay for monitoring the herbicide active ingredient glyphosate in water

Takács Eszter and Gémes Borbála and Szendrei Fanni and Keszei Csaba and Barócsi Attila and Lenk Sándor and Domján László and Mörtl Mária and Klátyik Szandra and Székács András: Enzyme-linked fluorescent immunoassay for monitoring the herbicide active ingredient glyphosate in water.

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Abstract

Within a modular water quality assessment fluorimeter instrument family, a newly developed enzyme-linked fluorescent immunoassay has been utilized for the quantitative analytical measurement of the herbicide active ingredient glyphosate in surface water. The developed 96- well microplate-based competitive immunoassay with fluorescence detection provides a 2.5- fold lower limit of detection (LOD = 0.09 ng/mL) in the investigated concentration range of glyphosate (0–100 ng/mL) compared to the detection of visual absorbance signals. Additionally, fluorescence detection resulted in a wider dynamic range for glyphosate measurement. Matrix effect was not observed for the undiluted surface water samples, and cross-reaction was not detected between glyphosate and its main metabolite (Naminomethylphosphonic acid) and structurally similar compounds. The method allows rapid monitoring of glyphosate as a ubiquitous water contaminant of agricultural origin that can affect, due to its global use, both aquatic and terrestrial ecosystems.

Item Type: Conference or Workshop Item
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2022
Volume: 28
ISBN: 978-963-306-904-2
Page Range: pp. 350-354
Language: English
Place of Publication: Szeged
Event Title: 28th International Symposium on Analytical and Environmental Problems
Event Type: Conference
Event Location: Szeged
Event Dates: 2022. november 14-15.
Related URLs: https://acta.bibl.u-szeged.hu/78308/
Uncontrolled Keywords: Analitikai kémia
Additional Information: Bibliogr.: 354. p. ; ill. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.04. Chemical sciences
Date Deposited: 2023. Mar. 24. 06:50
Last Modified: 2023. Mar. 24. 06:50
URI: http://acta.bibl.u-szeged.hu/id/eprint/78561

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