Characterization of hybrid chondroitin/dermatan sulfate octasaccharide domains in human brain by ion mobility tandem mass spectrometry

Ica Raluca and Sarbu Mirela and Biricioiu Roxana and Crumpei Ana-Maria and Sharon Edie and Clemmer David E. and Zamfir Alina D.: Characterization of hybrid chondroitin/dermatan sulfate octasaccharide domains in human brain by ion mobility tandem mass spectrometry.

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Abstract

We report here on the introduction of a rapid, highly sensitive and reliable approach in a single run, based on ion mobility separation (IMS), high resolution and tandem MS (MS/MS) by collision-induced dissociation (CID) for compositional and structural elucidation of neural chondroitin sulfate (CS) and dermatan sulfate (DS) domains, which implies the determination of the epimerization and the sulfation code of regular and irregular structures. By IMS MS and CID MS/MS, we were able to characterize in details CS/DS octasaccharides from brain obtained after CS/DS chain depolymerization by chondroitin B lyase and to detect sequences that were never found before in the octasaccharide domains of the investigated CS/DS brain fraction.

Item Type: Conference or Workshop Item
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2022
Volume: 28
ISBN: 978-963-306-904-2
Page Range: pp. 386-387
Language: English
Place of Publication: Szeged
Event Title: 28th International Symposium on Analytical and Environmental Problems
Event Type: Conference
Event Location: Szeged
Event Dates: 2022. november 14-15.
Related URLs: https://acta.bibl.u-szeged.hu/78308/
Uncontrolled Keywords: Elektrokémia
Additional Information: Bibliogr.: 387. p. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.04. Chemical sciences
Date Deposited: 2023. Mar. 24. 09:28
Last Modified: 2023. Mar. 24. 09:28
URI: http://acta.bibl.u-szeged.hu/id/eprint/78575

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