Fractionated high-pressure extraction of polyphenols from elderberry by-product

Nastić Nataša and Fernández Naiara and Živković Jelena and Mutavski Zorana and Milić Anita and Šavikin Katarina and Vidović Senka: Fractionated high-pressure extraction of polyphenols from elderberry by-product.

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The main focus of this study was to propose new streams to use plant waste, specifically to put on the demonstration of the possibility to implement emerging, green and safe technology into food and pharmaceutical industries in order to enable more economical waste management. For the isolation of valuable compounds from elderberry press cake (by-product left after pressing of juice), solvents and techniques considered as “acceptable” and “generally regarded as safe” in the food and pharmaceutical industries have been applied. Polyphenols rich elderberry press cake extracts were obtained employing a fractioned high-pressure methodology, supercritical fluid (SFE) and enhanced solvent extractions (ESE). SFE-CO2+ESE comprising a first SFECO2 step in order to remove the low polarity CO2-soluble compounds, and a second ESE step in order to extract polar compounds like anthocyanins have been carried out, as well as ESE without any pretreatment. The process kinetics of the extraction and increase of extraction yields (EY) were monitored at time intervals from 10 to 90 min, while EYs of ESE were measured after 30-220 min of extraction. Higher extract yield occurred in extract obtained by SFE-CO2+ESE (28.70%) showing its advantage over ESE. According to the kinetics results for the two-step extraction approach, extraction time of 100 min might be sufficient to achieve the similar final EY obtained after 220 min. HPLC of obtained extracts was utilized for quantification of polyphenolic compounds, showing cyanidin-3-O-sambubioside as a main compound, followed by rutin and cyanidin-3-O-glucoside. This work is the first to produce anthocyanin-rich fractions from black elderberry press cake using such high-performance technique.

Item Type: Conference or Workshop Item
Journal or Publication Title: Proceedings of the International Symposium on Analytical and Environmental Problems
Date: 2023
Volume: 29
ISBN: 978-963-306-963-9
Page Range: pp. 245-249
Language: English
Publisher: University of Szeged
Place of Publication: Szeged
Event Title: 29th International Symposium on Analytical and Environmental Problems
Event Type: Conference
Event Location: Szeged
Event Dates: 2023. november 13-14.
Related URLs:
Uncontrolled Keywords: Polifenolok, Extrakció
Additional Information: Bibliogr.: 249. p. ; ill. ; összefoglalás angol nyelven
Subjects: 01. Natural sciences
01. Natural sciences > 01.04. Chemical sciences
Date Deposited: 2023. Nov. 23. 15:18
Last Modified: 2023. Nov. 23. 15:18

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