Potential of Xanthomonas strains isolated in Serbia for Xanthan production from crude glycerol

Zahović Ida and Dodić Jelena and Trivunović Zorana: Potential of Xanthomonas strains isolated in Serbia for Xanthan production from crude glycerol.

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Abstract

Xanthan is a natural polysaccharide that is biosynthesized by metabolic activity of Gramnegative bacteria from the genus Xanthomonas. Commercial production of xanthan is mainly performed as aerobic submerged batch cultivation of the reference strain X. campestris ATCC 13951 on a medium of appropriate composition and under optimal conditions. The success of xanthan production is largely influenced by the selection of producing strains, formulation of cultivation medium composition, and control and regulation of bioprocess parameters. The aim of this study was to examine xanthan production on medium containing crude glycerol from domestic biodiesel industry using reference strain Xanthomonas campestris ATCC 13951 and Xanthomonas strains locally isolated from different vegetable cultures. Xanthan was produced by submerged cultivation of examined producing strains at a laboratory level under aerobic conditions at 30°C and 150 rpm for 168 h. Bioprocess efficacy was estimated based on the xanthan concentration in media at the end of biosynthesis. According to the obtained results, xanthan production on crude-glycerol based medium was possible by all applied Xanthomonas strains. The highest potential for successful xanthan production has PL4 strain, while strain Xp 3-1 has the lowest ability to biosynthesize xanthan in applied experimental conditions.

Item Type:	Conference or Workshop Item
Journal or Publication Title:	Proceedings of the International Symposium on Analytical and Environmental Problems
Date:	2023
Volume:	29
ISBN:	978-963-306-963-9
Page Range:	pp. 352-355
Language:	English
Publisher:	University of Szeged
Place of Publication:	Szeged
Event Title:	29th International Symposium on Analytical and Environmental Problems
Event Type:	Conference
Event Location:	Szeged
Event Dates:	2023. november 13-14.
Related URLs:	https://acta.bibl.u-szeged.hu/82256/
Uncontrolled Keywords:	Biotechnológia, Biopolimer
Additional Information:	Bibliogr.: 355. p. ; ill. ; összefoglalás angol nyelven
Subjects:	01. Natural sciences 01. Natural sciences > 01.04. Chemical sciences
Date Deposited:	2023. Nov. 27. 15:31
Last Modified:	2023. Nov. 27. 15:31
URI:	http://acta.bibl.u-szeged.hu/id/eprint/82476

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