Sarbu Mirela and Ica Raluca and Biricioiu Roxana and Clemmer David E. and Zamfir Alina D.: Identification and structural characterization of chondroitin sulfate disaccharides in human biglycan by nanoelectrospray ionization-ion mobility tandem mass spectrometry.
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Abstract
We have implemented a superior glycomics method based on ion mobility separation (IMS) mass spectrometry (MS) and tandem MS (MS/MS) to characterize the chondoritin sulfate (CS) disaccharide domains in human biglycan (BGN). The high separation efficiency and sensitivity of IMS MS technique allowed the discrimination of five distinct CS motifs, of which four irregulated in their sulfation pattern. The structural investigation by IMS MS/MS disclosed that in one or both of the CS/DS chains, the non-reducing end is 3-O-sulfated GlcA in a rare bisulfated motif having the structure 3-O-sulfated -GlcA-4-O-sulfated GalNAc. Considering the role played by BGN in cancer cell spreading, the influence on this process of the newly identified sequences is to be investigated in the future.
| Item Type: | Conference or Workshop Item |
|---|---|
| Journal or Publication Title: | Proceedings of the International Symposium on Analytical and Environmental Problems |
| Date: | 2024 |
| Volume: | 30 |
| ISBN: | 978-963-688-009-5 |
| Page Range: | pp. 223-224 |
| Language: | English |
| Publisher: | University of Szeged |
| Place of Publication: | Szeged |
| Event Title: | 30th International Symposium on Analytical and Environmental Problems |
| Event Type: | Conference |
| Event Location: | Szeged |
| Event Dates: | 2024. október 7-8. |
| Related URLs: | https://acta.bibl.u-szeged.hu/85607/ |
| Uncontrolled Keywords: | Biokémia, Tömegspektrometria |
| Additional Information: | Bibliogr.: 224. p. ; összefoglalás angol nyelven |
| Subjects: | 01. Natural sciences 01. Natural sciences > 01.04. Chemical sciences |
| Date Deposited: | 2024. Nov. 25. 08:55 |
| Last Modified: | 2024. Nov. 25. 08:55 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/85719 |
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