Gajdács Márió; Urbán Edit; Pallós Péter; Márta Adrienn; Matusovits Danica; Kárpáti Krisztina; Battah Basem; Hetta Helal F.; Khusro Ameer; Dordevic Dani; Kushkevych Ivan: Phenotypic characteristics of environmental Pseudomonas aeruginosa: an in vitro study on epidemiological aspects. In: Acta biologica Szegediensis, (67) 1. pp. 35-44. (2023)
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Pseudomonas aeruginosa is one of the most commonly isolated species among non-fermenting Gram-negative bacteria, both from clinical samples and from environmental sources. The survival of the species in harsh conditions is ensured by the production of a protective biofilm; assessment of biofilm-forming capacity aids future pathogen eradication strategies. The aim of our present study was to assess the relationship between antibiotic resistance, biofilm-forming capacity and other phenotypic virulence factors in environmental P. aeruginosa isolates. One hundred and fourteen (n = 114) isolates were included in the study, which were obtained from various geographical regions and environmental origins. Antimicrobial susceptibility testing was carried out using standard protocols. Biofilm-forming capacity and pyocyanin pigment production were tested using microtiter plate-based methods. Swarming, swimming and twitching motility, and siderophore-production were assessed using agar-plate based methodologies. Resistance in environmental isolates were highest for levofloxacin/ciprofloxacin 49.12% (n = 56), ceftazidime 42.98% (n = 49) and cefepime 35.96% (n = 41), while lowest for colistin 0% (n = 0); overexpression of RND-type efflux pumps was seen in 33.33% (n = 33) of isolates. 21.93% (n = 25) met the criteria to be classified as multidrug resistant (MDR). 17.54% (n = 20) of isolates were weak/non-biofilm producers, while (25.45%, n = 29) and (57.01%, n = 65) were moderate and strong biofilm producers, respectively. No significant differences were noted in biofilm-formation (OD570 values non-MDR [mean ± SD]: 0.396 ± 0.138 vs. MDR: 0.348 ± 0.181; p > 0.05) or pyocyanin pigment production (OD686 values non-MDR: 0.403 ± 0.169 vs. MDR: 0.484 ± 0.125; p > 0.05) between MDR and non-MDR environmental P. aeruginosa. Highest motility values were observed for swarming motility, followed by swimming and twitching motility; no relevant differences (p > 0.05) in motility were noted in the context of MDR status or biofilm-formation in the tested isolates. P. aeruginosa is an opportunistic pathogen with high medical importance, being a causative agent of recalcitrant infections, which are becoming difficult to treat with the onset of MDR. Further studies are warranted to assess biofilm-forming capacity, and to provide insights into the mechanisms underlying biofilm-formation both in isolates of clinical and environmental origins.
| Mű típusa: | Cikk, tanulmány, mű |
|---|---|
| Befoglaló folyóirat/kiadvány címe: | Acta biologica Szegediensis |
| Dátum: | 2023 |
| Kötet: | 67 |
| Szám: | 1 |
| ISSN: | 1588-4082 |
| Oldalak: | pp. 35-44 |
| Nyelv: | angol |
| Kiadó: | University of Szeged |
| Kiadás helye: | Szeged |
| Befoglaló mű URL: | https://acta.bibl.u-szeged.hu/86899/ |
| DOI: | 10.14232/abs.2023.1.35-44 |
| Kulcsszavak: | Mikrobiológia, Fertőző betegségek, Gyógyszerrezisztencia - mikrobák, Antibiotikum-ellenállás, Bakteriológia |
| Megjegyzések: | Bibliogr.: p. 40-44. ; összefoglalás angol nyelven |
| Szakterület: | 01. Természettudományok 01. Természettudományok > 01.06. Biológiai tudományok 03. Orvos- és egészségtudomány 03. Orvos- és egészségtudomány > 03.01. Általános orvostudomány 03. Orvos- és egészségtudomány > 03.03. Egészségtudományok |
| Feltöltés dátuma: | 2025. ápr. 15. 08:50 |
| Utolsó módosítás: | 2025. ápr. 15. 08:50 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/86936 |
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