Ormai Edit; Kocsis Béla; Böszörményi Andrea; Horváth Gyöngyi; Balázs Viktória Lilla: A teafa illóolaj biofilm-képződést gátló hatása kórházi környezetben előforduló baktériumok ellen. In: Móra Akadémia : szakkollégiumi tanulmánykötet 14., (14). pp. 99-110. (2025)
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Bacteria causing nosocomial infections are characterized by rapidly developing antibiotic resistance and the ability to form bioflms. Tese properties increase their resistance. Another characteristic is their ability to settle on inert surfaces which enhances the possibility of infections in immunosuppressed patients. Terefore it is important to explore alternative solutions to fght against these resistant pathogen in clinical practice. In our in vitro experiments we examined one essential oil and four bacteria. Based on literature data and preliminary results from our research group the essential oil of Melaleuca alternifolia (tea tree oil) is proved to be the most efective against these bacteria. We performed our research using trea tree oil. Based on clinical experiences four bacteria were selected which were most commonly isolated from biological samples (Escherichia coli, Klebsiella pneumoniae, Streptococcus pneumoniae, and Staphylococcus aureus). Our aim was to fnd out which bacteria are the most sensitive and which are the most resistant to the treatment. To determine the exact composition of tea tree oil analytical test (GCMS) was performed. Te next were the microbiological experiments. We determined the minimal inhibitory concentration (MIC) of tea tree oil using the microdilution method. Bioflms formed by bacteria were grown on 96-well microtiter plates, treated with half of the previously determined MIC concentration, and incubated. Te bioflms were fxed, stained, and absorbance was measured at 590 nm to specify the inhibitory efect of the essential oils on bioflm formation. According to literature data the main component of tea tree oil is terpinen-4-ol. Our fndings confrmed that the essential oil we used also contains it in the highest proportion (38%). All four bioflms created by the bacteria we examined were disrupted by the oil treatment. S. aureus was the most resistant to the treatment while S. pneumoniae was the most sensitive. Our results were also visualized using scanning electronmicroscopy for MRSA bacteria. Based on our in vitro results it can be concluded that tea tree essential oil was efective against bioflms formed by all four bacteria. Our further goal is to formulate a surface disinfectant solution to prevent the spreading of these agents with patients, thereby preventing the development and spread of infection.
| Mű típusa: | Könyv része |
|---|---|
| Egyéb cím: | Effect of Melaleuca alternifolia (tea tree) essential oil against nosocomial biofilm-forming bacteria |
| Rovatcím: | Biológia |
| Befoglaló folyóirat/kiadvány címe: | Móra Akadémia : szakkollégiumi tanulmánykötet 14. |
| Dátum: | 2025 |
| Kötet: | 14 |
| ISSN: | 2064-809X |
| Oldalak: | pp. 99-110 |
| Sorozat neve: | Móra Akadémia |
| Nyelv: | magyar , angol |
| Kiadó: | Szegedi Tudományegyetem Móra Ferenc Szakkollégium |
| Kiadás helye: | Szeged |
| Befoglaló mű URL: | https://acta.bibl.u-szeged.hu/88338/ |
| Kulcsszavak: | Illóolaj - teafa - gyógyhatás, Bakteriológia |
| Megjegyzések: | Bibliogr.: 108. p. és a lábjegyzetekben ; összefoglalás angol nyelven |
| Szakterület: | 01. Természettudományok 01. Természettudományok > 01.06. Biológiai tudományok |
| Feltöltés dátuma: | 2025. aug. 08. 15:20 |
| Utolsó módosítás: | 2025. aug. 08. 15:20 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/88345 |
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