Nafaee Zeyad H.; Gyurcsik Béla; Peintler Gábor: The effect of the metal ions on the catalytic activity of TEM-1 β-lactamase.
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The kinetic parameters of TEM-1 β-lactamase promoted ampicillin hydrolysis were revisited. The hydrolytic process was described by the kinetic analysis of the set of catalytic progress curves recorded at multiple wavelengths, including various concentrations of the enzyme and substrate. The primary hydrolysis product of ampicillin was converted to a second product, needed also to be considered in the evaluation process. Thus, instead of the usual MichealisMenten formalism, the full set of the stoichiometric and rate equations were used in the calculations. We showed that the catalytic activity of TEM-1 β-lactamase was slightly decreased by the gradual increase of concentration of Hg(II). These catalytic progress curves could be fitted allowing the change of the active enzyme concentration, yielding the same kinetic parameter values as in the absence of Hg(II). However, we have found that Ni(II) and Cd(II) ions slightly promoted the enzyme activity. We suggest the binding of these metal ions to the β-lactam ring of antibiotics activating the substrate for the nucleophilic attack by the enzyme.
| Mű típusa: | Konferencia vagy workshop anyag |
|---|---|
| Befoglaló folyóirat/kiadvány címe: | Proceedings of the International Symposium on Analytical and Environmental Problems |
| Dátum: | 2025 |
| Kötet: | 31 |
| ISBN: | 978-963-688-078-1 |
| Oldalak: | pp. 38-41 |
| Nyelv: | angol |
| Kiadó: | University of Szeged |
| Kiadás helye: | Szeged |
| Konferencia neve: | 31th International Symposium on Analytical and Environmental Problems |
| Konferencia típusa: | Konferencia |
| Helyszin: | Szeged |
| Dátum: | 2025. október 13-14. |
| Befoglaló mű URL: | https://acta.bibl.u-szeged.hu/88570/ |
| Kulcsszavak: | Biokémia |
| Megjegyzések: | Bibliogr.: p. 40-41. ; ill. ; összefoglalás angol nyelven |
| Szakterület: | 01. Természettudományok 01. Természettudományok > 01.04. Kémiai tudományok |
| Feltöltés dátuma: | 2025. nov. 07. 09:59 |
| Utolsó módosítás: | 2025. nov. 07. 09:59 |
| URI: | http://acta.bibl.u-szeged.hu/id/eprint/88615 |
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