relation: http://acta.bibl.u-szeged.hu/56234/
title: Triptofán és bizonyos metabolitjainak koncentrációjának meghatározása Creutzfeldt-Jakob betegeknél = The assessment of concentrations of certain tryptophan metabolites in Creutzfeldt-Jakob disease
creator:  Cseh Edina
creator:  Nánási Nikolett
creator:  Veres Gábor
creator:  Klivényi Péter
creator:  Danics Krisztina
creator:  Vécsei László
creator:  Kovács Gábor
creator:  Zádori Dénes
description: The kynurenine (KYN) pathway (KP), also known as the route where more than 95% of the tryptophan (TRP) is metabolized, in its steps of catabolism forms different metabolites which contribute to the neuroprotective–neurodegenerative changes in central nervous system. For this reason, TRP metabolism is extensively studied in neurodegenerative diseases (Alzheimer’s disease, Parkinson’s disease, Huntington’s disease), where the neurologically active metabolite concentration changes are followed. Kynurenic acid (KYNA), which is an endogenous N-methyl-D-aspartate receptor (NMDAR) antagonist, is considered to be a neuroprotective agent. In the present study TRP, KYN and KYNA were determined from human serum and cerebrospinal fluid (CSF) of patients with Creutzfeldt-Jakob disease (CJD) and age- and gender-matched controls, using high performance liquid chromatography (HPLC) applying UV and fluorescent detectors. The developed method was optimized and validated according to the International Congress Harmonization Guidelines. The precision and recovery values ranged between 1.60-4.36%, 81.61-101.09%, respectively. There were no differences between the groups with regard all the measured metabolites. The application of the developed validated method enabled the simultaneous determination of certain metabolites of the KP of TRP metabolism, but no evident alterations were found in patients with CJD.
date: 2017
type: Konferencia vagy workshop anyag
type: NonPeerReviewed
format: part
language: hu
identifier: http://acta.bibl.u-szeged.hu/56234/1/proceedings_of_isaep_2017_385-389.pdf
identifier:    Cseh Edina;  Nánási Nikolett;  Veres Gábor;  Klivényi Péter;  Danics Krisztina;  Vécsei László;  Kovács Gábor;  Zádori Dénes:   Triptofán és bizonyos metabolitjainak koncentrációjának meghatározása Creutzfeldt-Jakob betegeknél = The assessment of concentrations of certain tryptophan metabolites in Creutzfeldt-Jakob disease.