relation: http://acta.bibl.u-szeged.hu/85737/ title: Novel fluorescent-labeled estradiol derivatives creator: Hlogyik Tamás creator: Vágvölgyi Máté creator: Labos Péter creator: Bózsity Noémi creator: Zupkó István creator: Hunyadi Attila creator: Mernyák Erzsébet subject: 01. Természettudományok subject: 01.04. Kémiai tudományok description: The overproduction of the female sex hormone 17β-estradiol (E2) can lead to various hormone-dependent diseases, including tumors. Due to its biological activity in reproductive tissues and the nervous system, understanding its mechanisms of action and protein interactions is of significant importance. Fluorescent labeling methods are becoming increasingly favored over radiochemical techniques for making E2 detectable, as they offer more environmentally friendly alternatives. We published recently E2–BODIPY conjugates, labeled at the phenolic hydroxy function of the steroid, that retained estrogenic activity despite modifying the estradiol structure. Here we explored certain organic dyes suitable for fluorescent labeling, focusing on compounds, which provide tunable optical properties, high photostability, and physiological stability. A rhodamine, a rosamine and an aza-BODIPY dye were conjugated to an estradiol derivative bearing a four carbon-long linker at its 3-hydroxy group. We emphasized green chemistry approaches, including supercritical fluid chromatography, one-pot reactions, and microwave-assisted methods. publisher: University of Szeged date: 2024 type: Konferencia vagy workshop anyag type: NonPeerReviewed format: part language: hu identifier: http://acta.bibl.u-szeged.hu/85737/1/proceedings_of_isaep_2024_278-282.pdf identifier: Hlogyik Tamás; Vágvölgyi Máté; Labos Péter; Bózsity Noémi; Zupkó István; Hunyadi Attila; Mernyák Erzsébet: Novel fluorescent-labeled estradiol derivatives. language: eng