%0 Conference Paper
%A  Hlogyik Tamás
%A  Vágvölgyi Máté
%A  Labos Péter
%A  Bózsity Noémi
%A  Zupkó István
%A  Hunyadi Attila
%A  Mernyák Erzsébet
%B 30th International Symposium on Analytical and Environmental Problems
%C Szeged
%D 2024
%F acta:85737
%I University of Szeged
%K Orvosbiológiai kutatás, Biokémia, Fluoreszcens technológia
%P 278-282
%T Novel fluorescent-labeled estradiol derivatives
%U http://acta.bibl.u-szeged.hu/85737/
%V 30
%X The overproduction of the female sex hormone 17β-estradiol (E2) can lead to various hormone-dependent diseases, including tumors. Due to its biological activity in reproductive tissues and the nervous system, understanding its mechanisms of action and protein interactions is of significant importance. Fluorescent labeling methods are becoming increasingly favored over radiochemical techniques for making E2 detectable, as they offer more environmentally friendly alternatives. We published recently E2–BODIPY conjugates, labeled at the phenolic hydroxy function of the steroid, that retained estrogenic activity despite modifying the estradiol structure. Here we explored certain organic dyes suitable for fluorescent labeling, focusing on compounds, which provide tunable optical properties, high photostability, and physiological stability. A rhodamine, a rosamine and an aza-BODIPY dye were conjugated to an estradiol derivative bearing a four carbon-long linker at its 3-hydroxy group. We emphasized green chemistry approaches, including supercritical fluid chromatography, one-pot reactions, and microwave-assisted methods.
%Z Bibliogr.: 282. p. ; ill. ; összefoglalás angol nyelven