TY - CONF AV - public VL - 30 ID - acta85737 EP - 282 N1 - Bibliogr.: 282. p. ; ill. ; összefoglalás angol nyelven T2 - 30th International Symposium on Analytical and Environmental Problems TI - Novel fluorescent-labeled estradiol derivatives SN - 978-963-688-009-5 KW - Orvosbiológiai kutatás KW - Biokémia KW - Fluoreszcens technológia UR - http://acta.bibl.u-szeged.hu/85737/ M2 - Szeged N2 - The overproduction of the female sex hormone 17?-estradiol (E2) can lead to various hormone-dependent diseases, including tumors. Due to its biological activity in reproductive tissues and the nervous system, understanding its mechanisms of action and protein interactions is of significant importance. Fluorescent labeling methods are becoming increasingly favored over radiochemical techniques for making E2 detectable, as they offer more environmentally friendly alternatives. We published recently E2?BODIPY conjugates, labeled at the phenolic hydroxy function of the steroid, that retained estrogenic activity despite modifying the estradiol structure. Here we explored certain organic dyes suitable for fluorescent labeling, focusing on compounds, which provide tunable optical properties, high photostability, and physiological stability. A rhodamine, a rosamine and an aza-BODIPY dye were conjugated to an estradiol derivative bearing a four carbon-long linker at its 3-hydroxy group. We emphasized green chemistry approaches, including supercritical fluid chromatography, one-pot reactions, and microwave-assisted methods. Y1 - 2024/// CY - Szeged PB - University of Szeged SP - 278 A1 - Hlogyik Tamás A1 - Vágvölgyi Máté A1 - Labos Péter A1 - Bózsity Noémi A1 - Zupkó István A1 - Hunyadi Attila A1 - Mernyák Erzsébet ER -