Isolation and characterization of carbendazim-degrading bacteria from agricultural soil samples

Vágvölgyi Csaba; Németh V.; Sajben Enikő; Škrbić Biljana; Đurišić-Mladenović Nataša; Krisch Judit; Manczinger László: Isolation and characterization of carbendazim-degrading bacteria from agricultural soil samples. In: Agrár- és vidékfejlesztési szemle, (6) 1. pp. 320-324. (2011)

Cikk, tanulmány, mű agrarszemle_2011_001_suppl_320-324.pdf Letöltés (62kB)

Absztrakt (kivonat)

The use of chemical pesticides in agriculture generates many ecological and human toxicological problems. One of the most frequently used fungicides is carbendazim, however, in spite of its importance, there are only a few reports dealing with its microbial degradation in the environment. It has high acute ecotoxicological effect, as well as a suspected endocrine disruptor potential, so its residues in food and feed are dangerous. Until now, single isolates of Pseudomonas, Rhodococcus and Ralstonia have been found to be able to degrade carbendazim. Among fungi, one isolate of Alternaria alternata and Phanaerochete crysosporium were described as good carbendazim degraders. Bacterial degradation pathways have been partially explored: the first step is the hydrolysis of the carbamate group, followed by a ring-fission in 2-aminobenzimidazole resulting 1,2-diaminobenzene. This compound is further metabolized via the beta-ketoadipic acid pathway. As part of our studies on pesticide biodegradation, new carbendazim-metabolizing bacteria were isolated from Hungarian agricultural soil samples. These degrader bacteria were isolated from soil samples by microbiological enrichment methods. The molecular analysis revealed that the best isolates belong to the Variovorax paradoxus species. The isolate 10/1 was able to use carbendazim as sole carbon and nitrogen source. The pH optimum and temperature optimum for growth were found to be pH 6.3 and 30 °C, respectively. This isolate seems to be an efficient tool for the bioremediation of carbendazim polluted agricultural soils.

Tétel nézet