Characterization of serratia species and qualitative detection of serratia marcescens in raw and pasteurized milk by an analytical method based on polymerase chain reaction

Korcz Evelin and Varga László and Kerényi Zoltán: Characterization of serratia species and qualitative detection of serratia marcescens in raw and pasteurized milk by an analytical method based on polymerase chain reaction. In: Élelmiszervizsgálati közlemények, (67) 2. pp. 3453-3464. (2021)

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Abstract

Serratia species are opportunistic pathogenic microorganisms primarily known as nosocomial infectious agents, which can also cause food quality problems. The appearance of the extracellular pigment-producing Serratia marcescens in cow’s milk causes its red discoloration, posing a challenge to the dairy industry and food certification laboratories. The detection of the bacterium by conventional procedures based on microbiological methods is time-consuming and labor-intensive, and in many cases does not lead to satisfactory results due to the competitive inhibitory effect of the accompanying microflora. Following the analysis of the relevant literature, the published endpoint PCR methods and the primers used for the detection of S. marcescens were evaluated in in silico and in vitro assays, and then the procedure was tested on farm milk samples. Using the method, a total of 60 raw and pasteurized milk samples were analyzed, more than half of which (i.e., 32) were identified as S. marcescens positive. The significance of our work is mainly represented by the application of the published test methods in food industry practice. Our results highlight to the importance of detecting this bacterial species.

Item Type: Article
Other title: Serratia fajok jellemzése, valamint Serratia marcescens kvalitatív kimutatása nyers és pasztőrözött tejből polimeráz láncreakción alapuló vizsgálati módszerrel
Journal or Publication Title: Élelmiszervizsgálati közlemények
Date: 2021
Volume: 67
Number: 2
ISSN: 0422-9576
Page Range: pp. 3453-3464
Language: English
Publisher: WESSLING Nemzetközi Kutató és Oktató Központ Közhasznú Nonprofit Kft.
Place of Publication: Budapest
Related URLs: http://acta.bibl.u-szeged.hu/79017/
DOI: 10.52091/EVIK-2021/2-4-ENG
Uncontrolled Keywords: Mikrobiológia - módszer - élelmiszeripar
Additional Information: Bibliogr.: p. 3461-3464. ; ill. ; összefoglalás angol nyelven
Subjects: 02. Engineering and technology
02. Engineering and technology > 02.10. Other engineering and technologies
02. Engineering and technology > 02.10. Other engineering and technologies > 02.10.02. Food and beverages
Date Deposited: 2023. Jul. 13. 12:55
Last Modified: 2023. Aug. 26. 08:05
URI: http://acta.bibl.u-szeged.hu/id/eprint/79424

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